How does Qubit measure RNA?

How does Qubit measure RNA?

The Qubit® RNA HS Assay enables selective quantitation in the standard detection range of 5 – 100 ng total mass of RNA when using 1 – 20 μL in 200 μL assay volume. This equates to measuring RNA initial sample concentrations of 250 pg/μL – 100 ng/μL when using 1 – 20 μL of sample in 200 μL assay volume.

What is Qubit DNA?

Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.

What is Qubit concentration?

Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts.

Do Qubit reagents expire?

– The kit has expired or has been stored incorrectly: When properly stored, the components of the Qubit Protein Assay Kit should be stable for at least 6 months. Upon receipt, the kit can be stored at 4 degrees C.

How does qubit DNA work?

The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.

What does Qubit measure?

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.

What is the difference between Qubit and Nanodrop?

Devices using DNA binding dyes such as the qubit measure DNA concentrations directly, whereas the nanodrop quantifies anything that fluoresces at the approximate wavelength of DNA.

Is Qubit more sensitive than Nanodrop?

The sensitivity of the Qubit can be as low as 10 pg/mL, which is far superior to the Nanodrop. So, if you worry your samples have very low concentrations, it is best to use the Qubit.

Does Qubit use PicoGreen?

The Quant-iT PicoGreen dsDNA assay is well-adapted to high-throughput use, typically in a 96-well plate. Qubit dsDNA assay kits are convenient for fewer samples, since the samples are read one at a time. The Qubit assays are available as a HS (high sensitivity) assay kit and a BR (broad range) assay kit.

How does the Qubit DNA assay kit work?

The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer.

Which is most accurate qubit or dsDNA assay?

The assays are accurate for initial sample concentrations from as little as 250 pg/μL to 10,000 ng/μL. These kits are highly selective for RNA over double-stranded DNA (dsDNA). Unlike other RNA assays, they do not require DNase if DNA is present in the sample for an accurate measurement.

How are qubits used in nucleic acid quantification?

Sensitive and specific nucleic acid quantification assays. The Qubit assays utilize target-selective dyes that emit fluorescence when bound to DNA, RNA or protein. Unlike UV absorbance, which can overestimate sample concentrations due to contaminants in the sample such as salts, solvents, detergents, proteins, free nucleotides.

What are the components of qubit dsDNA BR?

Qubit dsDNA BR Standard #1 (Component C) 1 mL 5 mL 0 ng/µL in TE buffer ≤4°C Qubit dsDNA BR Standard #2 (Component D) 1 mL 5 mL 100 ng/µL in TE buffer Introduction The Qubit dsDNA BR (Broad-Range) Assay Kits make DNA quantitation easy and accurate. The kits include concentrated assay reagent, dilution buffer, and prediluted DNA standards.