What does brilliant blue stain?
Coomassie brilliant blue (CBB) stain is a widely used method for routine visualization of proteins separated on polyacrylamide gels. It is an organic dye that makes complexes with basic amino acids, such as lysine, histidine, tyrosine, and arginine.
Is Brilliant blue the same as Coomassie?
Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie Brilliant Blue G-250 differs from Coomassie Brilliant Blue R-250 by the addition of two methyl groups.
What is blue G?
Brilliant Blue G is used for staining protein(s) in polyacrylamide gels. It is also a P2X7 purinergic receptor antagonist. The receptor is mainly involved in neuroinflammatory events.
What does Coomassie Brilliant Blue bind to?
CBB binds to basic amino acids by a combination of hydrophobic interactions and heteropolar bonding, forcing proteins to move toward the anode, independently of their intrinsic pI.
What is the difference between R250 and G250 Coomassie Brilliant Blue G-250?
The more popular is Coomassie R250 (Reddish tinted blue) for electrophoresis (more sensitive: can detect as little as 0.1 µg of protein), and Coomassie G250 (Greenish tinted blue) for protein assay in solutions (because it is more convenient – soluble).
How do you cook Coomassie brilliant blue R250?
Dissolve 0.25 g of Coomassie Brilliant Blue R-250 in 90 ml of methanol:H2O (1:1, v/v) and 10 ml of glacial acetic acid. Filter the solution through a Whatman No. 1 filter to remove any particulate matter.
Is Coomassie Blue toxic?
Coomassie Briliant Blue R-250 Dye (6104-59-2) Potential Adverse human health effects and symptoms : Non-toxic if swallowed (LD50 oral, rat > 5000 mg/kg). Not irritant to skin. Not irritant to eyes.
What is Coomassie Brilliant Blue G 250?
Thermo Scientific Pierce Coomassie Brilliant Blue G-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).
What is the difference between R250 and G250 Coomassie Brilliant Blue G 250?
How do you cook Coomassie brilliant blue r250?
Can Coomassie blue go down the drain?
COOMASSIE BLUE destain may contain alcohol* and cannot be disposed of in the sink! Hazardous Materials are NEVER to be disposed of in the sink!
Where can I buy Coomassie Brilliant Blue G-250 dye?
ERR#1200 – This item cannot be ordered online. Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific Pierce Coomassie Brilliant Blue G-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.
What kind of stain is brilliant blue G?
BBG is a blue stain that is also known as acid blue 90 and Coomassie brilliant blue G 250. BBG has been used for protein staining in biological testing because it binds to proteins nonspecifically.
Are there any adverse reactions to brilliant blue G?
Adverse reactions that have been reported in procedures that included the use of Brilliant Blue G Ophthalmic Solution have often been associated with the surgical procedure. These complications include retinal (retinal break, tear, hemorrhage, and detachment) and cataracts. Included as part of the PRECAUTIONS section.
When was brilliant blue G approved by the FDA?
Brilliant blue G, like its name, imparts a vibrant blue color, facilitating identification of the ILM. It was approved by the FDA for ophthalmic use on December 20, 2019. Conformer generation is disallowed since too many atoms, MMFF94s unsupported element, mixture or salt