What is pcDNA used for?
pcDNA3. 0 is a mammalian expression vector of 5.4 kb which are specially designed for high-level stable and transient expression in mammalian hosts. pcDNA is available with the multiple cloning sites in the forward (+) and reverse (–) orientations to facilitate good cloning.
What is pcDNA3?
This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family.
What is a pCMV vector?
The pCMV-Script vector is derived from a high-copy-number pUC-based plasmid and is designed to allow protein expression in mammalian systems. Mammalian expression is driven by the human cytomegalovirus (CMV) immediate early promoter to promote constitutive expression of cloned inserts in a wide variety of cell lines.
What does Pcaggs stand for?
Definition. PCAG. Performance Confidence Assessment Group. PCAG. Primary Care Audit Group (healthcare; UK)
What is CMV GFP?
CMV-GFP lentivirus Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm).
What is UBC promoter?
The promoter of the polyubiquitin C gene (UBC) contains putative heat shock elements (HSEs) which are thought to mediate UBC induction upon stress. However, the mapping and the functional characterization of the cis-acting determinants for its up-regulation have not yet been addressed.
What is a .DNA file?
Data file created by SnapGene, a molecular biology program used for analyzing DNA data; stores a DNA sequence and associated XML and binary metadata; also includes the author, a description, comments, and other written information.
When was SnapGene created?
2004
Founded in 2004, SnapGene was born of frustration that founder Benjamin Glick, PhD, a faculty member in the University of Chicago’s Molecular Genetics and Cell Biology department, experienced with inefficiencies of DNA cloning in the lab.