What is Tre promoter?
The TRE is made up of Tet operator (tetO) sequence concatemers fused to a minimal promoter, (commonly the minimal promoter sequence derived from the human cytomegalovirus (hCMV) immediate-early promoter). In the absence of Tc or Dox, tTA binds to the TRE and activates transcription of the target gene.
What is pTRE plasmid?
pTRE-Tight is a response plasmid that can be used to express a gene of interest (Gene X) in our Tet-On® and Tet-Off® Gene Expression Systems and Cell Lines (1). cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively.
What is minimal CMV promoter?
Minimal CMV promoter expression plasmid that allows the insertion of transcription factor binding sites upstream of the minimal promtoer to create promoters with novel functions such as responsiveness to transciption factors or drugs. This allows tissue specific or physiologically responsive promoters to be created.
What is ef1a promoter?
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.
What is tetracycline induction?
Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline).
What is rtTA?
The reverse-tTA (rtTA) variant exhibits a reverse phenotype and does not bind tetO in the absence of an effector. Binding of dox triggers a conformational switch in rtTA, which allows tetO binding. Subsequent activation of the Ptet promoter drives expression of the downstream positioned gene.
What is the strongest promoter?
The two strongest promoters (clone 6 and clone 11) were investigated further Clone 6 turned out to be the strongest, showing a 3.0- and 8.4-fold activity in comparison to the two frequently used promoters–the cytomegalovirus (CMV) immediate early promoter and the simian virus 40 (SV40) early promoter respectively.
Is EF1a a strong promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
Is doxycycline a tetracycline drug?
Doxycycline is in a class of medications called tetracycline antibiotics. It works to treat infections by preventing the growth and spread of bacteria. It works to treat acne by killing the bacteria that infects pores and decreasing a certain natural oily substance that causes acne.
How is doxycycline different from tetracycline?
Acnecycline (Tetracycline) Treats bacterial infections. Vibramycin (doxycycline) is good for treating many bacterial infections, but can increase your skin’s sensitivity to sunlight and make you more likely to get a sunburn or rash. Kills bacteria and treats acne.
Does Tre tight promote doxycycline response?
The improved TRE promoter, TRE tight (Clontech) has been suggested to show a greater response to doxycycline coupled with extremely low basal transcriptional activity by redesign of the 7 tetO sequences that make up the TRE and the removal of the potential binding sites of endogenous transcription factors ( Clontechniques, 2003 ).
Why does TetR bind to tetracycline instead of Tre?
In the endogenous bacterial system, if tetracycline, or one of its analogs like doxycycline, are present, tetR will bind to tetracycline and not to the TRE, permitting transcription. Tetracycline-dependent promoters are developed by placing a TRE upstream of a minimal promoter.
Can doxycycline adipogenic inducible expression be achieved using Plenti Tre / rtTA ADV?
The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.
How are tetracycline-dependent promoters developed?
Tetracycline-dependent promoters are developed by placing a TRE upstream of a minimal promoter. The initial system Gossen and Bujard developed is known as tetracycline off: in the presence of tetracycline, expression from a tet-inducible promoter is reduced.