What does EDC do chemistry?

The chemical compound 1,2-dichloroethane, commonly known as ethylene dichloride (EDC), is a chlorinated hydrocarbon, mainly used to produce vinyl chloride monomer (VCM, chloroethene), the major precursor for PVC production. It is a colorless liquid with a chloroform-like odor.

Does EDC react with NHS?

EDC (carbodiimide) crosslinking reaction scheme. Carboxyl-to-amine crosslinking with the popular carbodiimide, EDC. EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O-acylisourea intermediate while allowing for efficient conjugation to primary amines at physiologic pH.

How do I dissolve EDC?

For KLH, dissolve 10mg EDC in 1ml deionized water and immediately add 50µl EDC solution to the protein:hapten solution. If precipitation occurs, reduce the amount of EDC solution added further.

Is lead a EDC?

While lead has been banned in house paints, dishes, and cookware in the United States since 1978, this EDC may still be found in a product’s paint – especially in products manufactured in countries which still allow lead-based paint – and in plastics where lead is still allowed for softening and stabilizing against …

What is the difference between DCC and EDC?

The key difference between DCC and EDC is that DCC is a cyclic compound, whereas EDC is an aliphatic compound. DCC and EDC are organic compounds. The term DCC stands for N,N′-Dicyclohexylcarbodiimide while the term EDC stands for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide.

Is EDC a carcinogen?

EPA has classified ethylene dichloride as a Group B2, probable human carcinogen.

Is lead an EDC?

What happens when EDC is mixed with carboxylic acid?

EDC reaction chemistry EDC reacts with carboxylic acid groups to form an active O-acylisourea intermediate that is easily displaced by nucleophilic attack from primary amino groups in the reaction mixture. The primary amine forms an amide bond with the original carboxyl group, and an EDC by-product is released as a soluble urea derivative.

How to coupling an amino acid with EDC?

Coupling with EDC 1 Dissolve the N-protected amino acid and the amino acid ester to be coupled in dichloromethane (DCM). 2 Cool the mixture in an ice bath. 3 Add 1.2 equivalents of EDC and stir the mixture. 4 When the reaction is complete, wash the mixture with water to remove excess EDC and urea by-product.

Which is an example of the EDC / NHS activation mechanism?

The EDC/NHS activation results for four small molecules of dicarboxylic acids in aqueous media, namely, glutaric acid and 2,2-dimethyl glutaric acid, which generate disuccinimidyl ester with high yield, and succinic acid and 2,2-dimethyl succinic acid, which remain intact, can also be explained by the Thorpe–Ingold effect.

What kind of activating agent is EDC used for?

It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds. Additionally, EDC can also be used to activate phosphate groups in order to form phosphomono-esters and phosphodiesters.