What does JC-1 stain?
JC-1 dye can be used as an indicator of mitochondrial potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria.
How JC1 works?
JC-1 as a fluorescent cationic carbocyanine dye exhibits potential-dependent accumulation in mitochondria, which form J aggregates and it diffuses across mitochondria to form monomeric state upon depolarization (Figure 1).
What is a JC-1 assay?
The MitoPT JC-1 Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation.
What is JC1?
JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The dye exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ~590 nm.
What is Annexin V staining?
Annexin V staining is a common method for detecting apoptotic cells. Thermo Fisher Scientific offers high-quality fluorescent annexin V conjugates as standalone reagents and in a variety of kits for use in flow cytometry and for imaging suspension cells.
How do you test mitochondrial membrane potential?
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells.
What does FCCP mean for a doctor?
Fellow of the American College of Chest Physicians
When you become an FCCP (Fellow of the American College of Chest Physicians), you play an active role in advancing the field of chest medicine while enjoying the prestige of being associated with a distinctive group of chest medicine professionals.
Can I fix annexin V stained cells?
Yes, cells can be fixed with formaldehyde after staining. Because Annexin V staining is dependent on calcium, all buffers used for washing and fixation should contain 1.25 mM CaCl2. Fixation may increase the background signal from Ethidium Homodimer III.
What does a decrease in mitochondrial membrane potential mean?
Withdrawal of growth factors or loss of the extracellular glucose supply will lead to a decline in mitochondrial membrane potential (MMP). If growth factor or glucose deprivation persists, cells ultimately undergo apoptosis that is initiated by cytochrome c release from mitochondria.
How to explain the use of JC1 dye?
How to explain? I treated cells (platelets) to initiate cell apoptosis and used JC1 dye to measure the mitochondrial membrane potential by flow cytometry.
How is JC-1 dye used in apoptosis studies?
JC-1 Dye for Mitochondrial Membrane Potential The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria.
How is JC-1 dye used for mitochondrial membrane potential?
JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. We offer Invitrogen JC-1 dye as a bulk chemical and in the Invitrogen MitoProbe JC-1 Assay Kit, optimized for flow cytometry.
How is JC-1 used in flow cytometry?
JC-1 dye is well known for using mitochondrial membrane potential disruption detection during apoptosis. JC-1 entry is from the cell membrane to the cytosol to the mitochondria and forms aggregates. In apoptotic cells these aggregates leak out from the mitochondria to the cytosol as monomers. A.