What would it mean if the molar extinction coefficient had a value of zero at a given wavelength?

An absorbance of 0 at some wavelength means that no light of that particular wavelength has been absorbed. The intensities of the sample and reference beam are both the same, so the ratio Io/I is 1 and the log10 of 1 is zero.

Can you have an absorbance over 1?

Absorbance values greater than or equal to 1.0 are too high. If you are getting absorbance values of 1.0 or above, your solution is too concentrated. Simply dilute your sample and recollect data . At an absorbance of 2 you are at 1%T, which means that 99% of available light is being blocked (absorbed) by the sample.

What are the two conditions that will make the molar extinction coefficient ε a constant?

Molar absorption coefficient (ε) Under defined conditions of solvent, pH and temperature the molar absorption coefficient for a particular compound is a constant at the specified wavelength.”

What is the LCSs formula for protoporphyrin?

Laboratory Chemical Safety Summary (LCSS) Datasheet. Molecular Formula. C34H34N4O4. Synonyms. protoporphyrin IX. protoporphyrin. 553-12-8. Ooporphyrin. Protoporpyrin IX.

What is protoporphyrin IX (PPIX)?

Protoporphyrin IX is an organic compound, specifically a porphyrin, that plays an important role in living organisms as a precursor to other critical compounds like hemoglobin and chlorophyll. It is a deeply colored solid that is not soluble in water. The name is often abbreviated as PPIX.

How does protoporphyrin IX react with iron salts?

Protoporphyrin IX reacts with iron salts in air to give the complex FeCl (PPIX). Heme coordinated with chlorine is known as hemin. Many metals other than Fe form Heme -like complexes when coordinated to PPIX. Of particular interest are cobalt derivatives because they also function as oxygen carriers.

How is protoporphyrin IX produced from methylene bridge?

Protoporphyrin IX is produced by oxidation of the methylene bridge of protoporphyrinogen by the enzyme protoporphyrinogen oxidase. Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue.